Iron chelation / Eale's disease

Discussion in 'Optometry Archives' started by tom hennessy, Nov 2, 2004.

  1. tom hennessy

    tom hennessy Guest

    Curr Eye Res. 2004;28(6):399-407. Related Articles, Links

    Iron chelation abrogates excessive formation of hydroxyl radicals and
    lipid peroxidation products in monocytes of patients with Eales'
    disease: Direct evidence using electron spin resonance spectroscopy.

    Rajesh M, Sulochana K, Ramakrishnan S, Biswas J, Manoharan P.

    Biochemistry Department Vision Research Foundation, Sankara Nethralaya
    Chennai India.

    Purpose. Eales' disease (ED) is an idiopathic retinal vasculitis
    condition, which affects the retina of young adult males. Retinal
    changes include perivasculitis, non-perfusion and neovascularization.
    Disruption of blood-retinal barrier (BRB) is the common feature in
    intra-ocular inflammatory diseases. Disruption of BRB results in
    vascular hyper permeability and infiltration of circulating leukocytes
    into the retinal parenchyma. Monocyte (MC) activation results in
    oxidant thrust and subsequent tissue damage. This has been reported in
    various intra-ocular inflammatory diseases such as uveitis and
    Behcet's disease. However, there are no such reports available in ED.
    Hence in the present study we have investigated the role of MC
    activation and hydroxyl radicals ((*)OH) production and its possible
    involvement in promoting the development of retinal vasculitis in
    patients with ED. Methods. Twelve patients with ED and twelve healthy
    volunteers were recruited for the study. MC was separated from their
    peripheral blood. MC from patients with ED and control subjects was
    stimulated with phorbol-12-myristate - acetate (PMA) and (*)OH
    generated was analyzed using an electron spin resonance spectrometer
    (ESR). Superoxide dismutase (SOD), thiobarbituric acid reactive
    substances (TBARS), and iron content was determined in MC to assess
    the oxidant thrust and antioxidant defense. Results. (*)OH generation
    was elevated in MC from patients with ED, which coincided with
    diminished SOD activity and elevated levels of iron and TBARS, when
    compared with healthy control subjects. (*)OH generation was abrogated
    when MC from ED were co-incubated with PMA and iron chelators such as
    diethylenetriaminepentacetic acid (DTPA) and desferrioxamine. Iron
    chelation also inhibited TBARS accumulation restored SOD activity in
    MC of patients with ED. Conclusions. For the first time we have
    demonstrated the production of (*)OH generation in MC of patients with
    ED using ESR. Further we have shown the beneficial effect of iron
    chelation in mitigating free radical mediated changes in cellular
    metabolism. Based on our findings, we provide further evidence for the
    role of oxidant thrust in promoting retinal tissue damage in patients
    with ED.

    PMID: 15512947 [PubMed - as supplied by publisher]
    tom hennessy, Nov 2, 2004
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